The national bus service announced Thursday that it was going to cease operation to Brattleboro starting June 20.
The company also said it was going to stop service to Bellows Falls, Keene, N.H., and Greenfield and Northampton in Massachusetts.
Greyhound Spokesman Timothy Stokes said the company decided to stop the New England service due to low ridership.
Stokes said buses will still travel to Springfield, Mass. and to White River Junction, but there will be no stops along Interstate 91 between those two cities.
Greyhound first pulled out of Brattleboro in April 2011, when the small station on Putney Road near exit 3 abruptly shut its doors with no explanation.
That decision was made by the local ticket agent and the company at the time was still serving Bellows Falls and Keene.
In June 2011 the Fleming Shell Station at 207 Canal St announced it would sell Greyhound tickets and service resumed in Brattleboro on June 7, 2011.
Now, about one year later, the company will once again stop driving its silver buses through town.
Rick Fleming, who owned the Shell Station at the time, said Greyhound approached him about becoming a ticket agent.
"We are doing this due to low ridership," Stokes said. "Every year we evaluate the routes and see where we should increase routes or decrease routes. At this point there are no other plans."