The Vermont State Police Bureau of Criminal Investigation earlier this week took the lead of this case.
According to reports, searchers have combed the area near Holmes' house and other locations where she may be, but have not found any results.
Investigators have also said that there has been no activity on her cellphone, social media or financial accounts.
Holmes, 59, is 5-foot, 9-inches tall and weighs 170 pounds. She has blonde hair and green eyes.
On the morning of Sept. 6, Holmes told her husband, Steven M. St. Peter, that she was going to tend to animals on their farm around 8 a.m. When he woke up around 10 a.m., there was a note on the butcher block that said she went for a ride.
"(It said) she'd see me later on Friday afternoon. She said, 'I love you,' but I haven't heard from her ever since," said St. Peter, who was the last person who has claimed to have seen her.
Investigators are speaking to friends and family, while still actively looking for her vehicle, a maroon 2010 Subaru Forester with license plates that read DAK316, registered in Vermont and due to expire in 2015.
Shortly after her disappearance, Helen's daughter, Chelsea L. Allard, posted on Facebook that "This is not a case of escaping for the weekend to blow off steam, this is a case of LIFE AND DEATH. ... We are not limiting the search to Vermont. She had hours of time to drive far away before she was reported missing. ... I want my mom back safe."
The police are concerned for her safety due to the fact she hasn't reached out to family or friends.
Holmes is a registered nurse at Grace Cottage Hospital. She was scheduled for work on Monday but did not show up. The hospital posted information about her being missing. The post also stated that she is a beloved nurse "responsible for arranging for the miniature horses to come to visit a Hospice patient at Grace Cottage last year."
Anyone who has seen or heard from Helen Holmes since the morning of the Sept. 6 is encouraged to contact the Vermont State Police at the Brattleboro Barracks at 802-254-2382. Or submit an anonymous an anonymous tip by texting "CRIMES" (274637) with keyword: VTIPS or online at http://vsp.vermont.gov/tipsubmit.